Studies on the nutrition of Brucella melitensis.
نویسندگان
چکیده
Koser, Breslove, and Doirfman (1941) obtained moderate growth (one billion cells per ml) of the three species, Brucella melitensis, Brucella abortus, and Brucella suis, in a chemically defined medium consisting of 17 amino acids, glucose, inorganic salts, thiamin, nicotinamide, biotin, and pantothenic acid. All of the strains studied required thiamin and nicotinamide, and all weere stimulated by pantothenic acid. Growth of B. abortus strains was stimulated by a biotin concentrate. McCullough et al. (1947) developed a chemically defined medium for B. suis consisting of 14 amino acids (5 of w-hich weie considered essential and 9 stimulatory), glucose, inorganic salts, thiamin, nicotinic acid, biotin, and pantothenate. Wtith this medium they obtained vields of approximately 35 billion cells per ml. With 12 amino acids and added purines they obtained more than 50 billion cells per ml. The nutritional requirements of brucellae also have been studied by many other investigators. The media employed, however, were either unduly complex because of the number of amino acids required in their pieparation oIr failed to produce a high y-ield of viable cells. The present investigation of the nutritional requirements of strain 4247 of B. melitensis w-as undertaken to devise a simple synthetic medium that would give abundant growth. A preliminary report of this work was presentedl by Sanders, Higuchi, and Brewer (1951).
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عنوان ژورنال:
- Journal of bacteriology
دوره 66 3 شماره
صفحات -
تاریخ انتشار 1953